QRFP Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11534R-RBITC-100uL |
| Product Name | QRFP Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human QRFP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted |
| Synonyms | 26RFa; Neuropeptide RF-amide; OX26_HUMAN; pyroglutamylated RFamide peptide; P518; Pyroglutamylated arginine-phenylalanine-amide peptide; QRF-amide; Qrfp. |
| Background | QRFP is a 136 amino acid secreted protein that is widely expressed in the brain with highest expression levels in the cerebellum, medulla, pituitary, retina, vestibular nucleus and white matter. Also expressed in the bladder, colon, coronary artery, parathyroid gland, prostate, testis and thyroid, QRFP is a member of the RFamide neuropeptide family. QRFP may be involved in aldosterone secretion by the adrenal gland and is also implicated in stimulating metabolic rate, locomotor activity and increasing blood pressure. Considered a ligand for the G-protein coupled receptor GPR103, QRFP may have orexigenic activity, thereby acting as an appetite stimulant. The gene encoding QFRP is located on human chromosome 9, which consists of about 145 million bases, comprises roughly 4% of the human genome and encodes nearly 900 genes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is QRFP Polyclonal Antibody, RBITC Conjugated (bs-11534R-RBITC) validated for, and at what dilution?
bs-11534R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does QRFP Polyclonal Antibody, RBITC Conjugated (bs-11534R-RBITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Pig, Horse.
How should bs-11534R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise QRFP Polyclonal Antibody, RBITC Conjugated (bs-11534R-RBITC)?
KLH conjugated synthetic peptide derived from human QRFP
What are the excitation and emission wavelengths of the RBITC conjugate (bs-11534R-RBITC)?
RBITC on bs-11534R-RBITC: excitation / emission 540nm/625nm.