Bid Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1153R-APC-100uL |
| Product Name | Bid Polyclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Bid |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 12122 |
| Swiss Prot | P70444 |
| Subcellular location | Cytoplasm |
| Synonyms | AI875481; AU022477; 2700049M22Rik; BH3-interacting domain death agonist; p22 BID; BID |
| Background | Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Bid Polyclonal Antibody, APC Conjugated (bs-1153R-APC) validated for, and at what dilution?
bs-1153R-APC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bid Polyclonal Antibody, APC Conjugated (bs-1153R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1153R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bid Polyclonal Antibody, APC Conjugated (bs-1153R-APC)?
KLH conjugated synthetic peptide derived from mouse Bid
What are the excitation and emission wavelengths of the APC conjugate (bs-1153R-APC)?
APC on bs-1153R-APC: excitation / emission 650nm/660nm.