FOXB1 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11555R-BF750-100uL |
| Product Name | FOXB1 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Sheep, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FOXB1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | FKH 5; FKH5; Forkhead box B1; Forkhead box protein B1; FOX B1; FOXB 1; HFKH 5; Transcription factor FKH 5; Transcription factor FKH5; FOXB1_HUMAN. |
| Background | The Forkhead-box (FOX) genes comprise a superfamily of at least 43 members that encode proteins which are involved in transcriptional regulation and may be associated with the pathogenesis of various cancers. FOXB1 (forkhead box B1), also known as FKH5 or HFKH-5, and FOXB2 (forkhead box B2) are members of the FOX family and each contain one forkhead DNA-binding domain. Both FOXB1 and FOXB2 localize to the nucleus where they are thought to function as transcription factors that can bind to DNA via their forkhead domains. In mice, defects in the gene encoding FOXB1 are associated with retarded development of the central nervous system (CNS), suggesting that FOXB1 may play a role in CNS organization and function. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FOXB1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11555R-BF750) validated for, and at what dilution?
bs-11555R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FOXB1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11555R-BF750) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Chicken.
How should bs-11555R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FOXB1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11555R-BF750)?
KLH conjugated synthetic peptide derived from human FOXB1
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-11555R-BF750)?
AbBy Fluor-750 on bs-11555R-BF750: excitation / emission 749nm/775nm.