ASCL1 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1155R-APC |
| Product Name | ASCL1 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ASCL1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 429 |
| Swiss Prot | P50553 |
| Subcellular location | Nucleus |
| Synonyms | ASH1; HASH1; MASH1; bHLHa46; Achaete-scute homolog 1; ASH-1; Class A basic helix-loop-helix protein 46; ASCL1 |
| Background | Transcription factor that controls transcriptional expression of its target genes by binding to the E box (5'-CANNTG-3'). Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Essential for the generation of olfactory and autonomic neurons (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ASCL1 Polyclonal Antibody, APC Conjugated (bs-1155R-APC) validated for, and at what dilution?
bs-1155R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ASCL1 Polyclonal Antibody, APC Conjugated (bs-1155R-APC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep.
How should bs-1155R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ASCL1 Polyclonal Antibody, APC Conjugated (bs-1155R-APC)?
KLH conjugated synthetic peptide derived from human ASCL1
What are the excitation and emission wavelengths of the APC conjugate (bs-1155R-APC)?
APC on bs-1155R-APC: excitation / emission 650nm/660nm.