RAPGEF5/Repac Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11582R-BF350-100uL |
| Product Name | RAPGEF5/Repac Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RAPGEF5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | GFR; Guanine nucleotide exchange factor for Rap1; KIAA0277; M Ras regulated Rap GEF; M-Ras-regulated Rap GEF; MR GEF; MR-GEF; MRGEF; Rap guanine nucleotide exchange factor 5; Rapgef5; Related to Epac; Repac; RPGF5_HUMAN. |
| Background | Members of the RAS subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as Repac, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation. Repac, also known as Rap guanine nucleotide exchange factor 5 (RAPGEF5), is a 580 amino acid protein that is expressed in brain and testis, with weaker expression in heart, placenta, lung, pancreas and small intestine. Existing as two alternatively spliced isoforms, the Repac gene is conserved in cow, mouse and rat. The gene that encodes Repac maps to human chromosome 7p15.3, with high expression of this region linking to positive response to erlotinib therapy for those with non-small-cell lung cancer. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RAPGEF5/Repac Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11582R-BF350) validated for, and at what dilution?
bs-11582R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11582R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAPGEF5/Repac Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11582R-BF350)?
KLH conjugated synthetic peptide derived from human RAPGEF5
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-11582R-BF350)?
AbBy Fluor-350 on bs-11582R-BF350: excitation / emission 346nm/442nm.