DISP2 Polyclonal Antibody, PerCP Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11588R-PerCP-100uL |
| Product Name | DISP2 Polyclonal Antibody, PerCP Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DISP2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 85455 |
| Swiss Prot | A7MBM2 |
| Subcellular location | Cell membrane |
| Synonyms | DISPB; HsT16908; Protein dispatched homolog 2; DISP2; KIAA1742 |
| Background | DISP2 is the second of three known homologs of the D.melanogaster protein Dispatched. It is a multitransmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored Hedgehog from producing cells. Hedgehog is a major player in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis and the DISP proteins have been implicated in these pathways. Recently, it has been shown that DISP2 is translationally regulated by the microRNA miR-214 in zebrafish. Expression of this miRNA decreased DISP2 promoter activity in vitro and its overexpression in zebrafish resulted in a phenotype identical to that observed by DISP2 mutants. At least two isoforms of DISP2 are known to exist. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DISP2 Polyclonal Antibody, PerCP Conjugated (bs-11588R-PerCP) validated for, and at what dilution?
bs-11588R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11588R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DISP2 Polyclonal Antibody, PerCP Conjugated (bs-11588R-PerCP)?
KLH conjugated synthetic peptide derived from human DISP2
What are the excitation and emission wavelengths of the PerCP conjugate (bs-11588R-PerCP)?
PerCP on bs-11588R-PerCP: excitation / emission 482nm/677nm.