HOXC5 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11589R-RBITC-100uL |
| Product Name | HOXC5 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HOXC5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | CP11; homeo box C5; homeobox C5; Homeobox protein CP11; homeobox protein Hox C5; Homeobox protein Hox-3D; Homeobox protein Hox-C5; Hox 3D; HOX3; HOX3D; HOXC5; HXC5_HUMAN; OTTHUMP00000217386. |
| Background | The Hox homeobox genes encode proteins that are transcriptional regulators with an established role in embryonic development. HoxC5 is a 222 amino acid protein that localizes to the nucleus and contains one homeobox DNA-binding domain. Existing as multiple alternatively spliced isoforms, HoxC5 functions as a sequence-specific DNA-binding transcription factor that is part of a regulatory mechanism that provides cells with positional identities during development. The gene encoding HoxC5 maps to a cluster of Hox proteins on chromosome 12 that are essential for morphogenesis. Encoding over 1,100 genes within 132 million bases, chromosome 12 makes up about 4.5% of the human genome. A number of skeletal deformities are linked to chromosome 12, including hypochondrogenesis, achondrogenesis and Kniest dysplasia. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HOXC5 Polyclonal Antibody, RBITC Conjugated (bs-11589R-RBITC) validated for, and at what dilution?
bs-11589R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HOXC5 Polyclonal Antibody, RBITC Conjugated (bs-11589R-RBITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep.
How should bs-11589R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HOXC5 Polyclonal Antibody, RBITC Conjugated (bs-11589R-RBITC)?
KLH conjugated synthetic peptide derived from human HOXC5
What are the excitation and emission wavelengths of the RBITC conjugate (bs-11589R-RBITC)?
RBITC on bs-11589R-RBITC: excitation / emission 540nm/625nm.