Zic1 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11609R-BF405 |
| Product Name | Zic1 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Zic1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Odd paired homolog Drosophila; Zic 1; ZIC; Zic family member 1 odd-paired Drosophila homolog; Zic family member 1; Zic protein member 1; zic1; ZIC1_HUMAN; Zinc finger protein 201; Zinc finger protein of the cerebellum 1; Zinc finger protein ZIC 1; Zinc finger protein ZIC1; ZNF 201; ZNF201. |
| Background | Zic1 is a C2H2 zinc finger transcription factor that controls the expansion of neuronal precursors by inhibiting the progression of neuronal differentiation. Zic1 determines the cerebellar folial pattern by influencing proliferation in the external germinal layer (EGL). Zic1 can bind and transactivate the apolipoprotein E gene.This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 4, a related family member on chromosome 3. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Zic1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11609R-BF405) validated for, and at what dilution?
bs-11609R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Zic1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11609R-BF405) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Pig, Chicken.
How should bs-11609R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Zic1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11609R-BF405)?
KLH conjugated synthetic peptide derived from human Zic1
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-11609R-BF405)?
AbBy Fluor-405 on bs-11609R-BF405: excitation / emission 401nm/421nm.