p35/CDK5P1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-11611r-cy55-100uL |
| Product Name | p35/CDK5P1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human p35 |
| Immunogen Range | 225-270/307 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8851 |
| Swiss Prot | Q15078 |
| Synonyms | p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a; Cyclin-dependent kinase 5 activator 1; CDK5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; TPKII regulatory subunit; CDK5R1 |
| Background | p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is p35/CDK5P1 Polyclonal Antibody, Cy5.5 Conjugated (BS-11611R-CY5-5) validated for, and at what dilution?
BS-11611R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p35/CDK5P1 Polyclonal Antibody, Cy5.5 Conjugated (BS-11611R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-11611R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise p35/CDK5P1 Polyclonal Antibody, Cy5.5 Conjugated (BS-11611R-CY5-5)?
KLH conjugated synthetic peptide derived from human p35 Immunogen range: 225-270/307.
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-11611R-CY5-5)?
Cy5.5 on BS-11611R-CY5-5: excitation / emission 675nm/694nm.