phospho-GluR1 (Ser645) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11633R-APC-Cy7-100uL |
| Product Name | phospho-GluR1 (Ser645) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human GluR1 around the phosphorylation site of Ser645 |
| Modification Site | Ser645 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | p-GluR-1S645; p-GluR1Ser645; Glutamate Receptor 1 phospho S645; p-Glutamate Receptor 1 phospho S645;;GLUR 1; GLUR A; AMPA 1; GluR-1; AMPA selective glutamate receptor 1; AMPA-selective glutamate receptor 1; GluA1; GLUH 1; GLUH1; GluR K1; GluR-1; GluR-A; GluR-K1; GLUR1; GLURA; GluRK1; Glutamate receptor 1; Glutamate receptor ionotropic AMPA 1; Glutamate receptor ionotropic; Glutamate receptor, ionotropic, AMPA 1; Gria 1; Gria1; GRIA1_HUMAN; HBGR1; MGC133252; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243. |
| Background | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-GluR1 (Ser645) Polyclonal Antibody, APC-Cy7 Conjugated (bs-11633R-APC-Cy7) validated for, and at what dilution?
bs-11633R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-GluR1 (Ser645) Polyclonal Antibody, APC-Cy7 Conjugated (bs-11633R-APC-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-11633R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-GluR1 (Ser645) Polyclonal Antibody, APC-Cy7 Conjugated (bs-11633R-APC-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human GluR1 around the phosphorylation site of Ser645
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-11633R-APC-Cy7)?
APC-Cy7 on bs-11633R-APC-Cy7: excitation / emission 650nm/780nm.