Contactin 4 + 6 Polyclonal Antibody, PerCP Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11636R-PerCP-100uL |
| Product Name | Contactin 4 + 6 Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Sheep, Pig, Chicken |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Contactin 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | AXCAM; axonal-associated cell adhesion molecule; BIG 2; BIG2; Brain-derived immunoglobulin superfamily protein 2; CNTN4; CNTN4A; CNTN4_HUMAN; CNTN6; CNTN6_HUMAN; Contactin 4; Contactin4; Contactin-4; Contactin-6; Contactin6; Contactin 6; hNB-3; NB3; neural cell adhesion protein BIG-2; Neural recognition molecule NB-3; Contactin-3; Brain-derived immunoglobulin superfamily protein 1; BIG-1; Plasmacytoma-associated neuronal glycoprotein; CNTN3; KIAA1496; PANG; Contactin-5; Neural recognition molecule NB-2; hNB-2; CNTN5 |
| Background | Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination. Tissue specificity: Expressed in nervous system. Highly expressed in cerebellum. Expressed at intermediate level in thalamus, subthalamic nucleus. Weakly expressed in corpus callosum, caudate nucleus and spinal cord. Similarity: Belongs to the immunoglobulin superfamily. Contactin family. Contains 4 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Contactin 4 + 6 Polyclonal Antibody, PerCP Conjugated (bs-11636R-PerCP) validated for, and at what dilution?
bs-11636R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11636R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Contactin 4 + 6 Polyclonal Antibody, PerCP Conjugated (bs-11636R-PerCP)?
KLH conjugated synthetic peptide derived from human Contactin 4
What are the excitation and emission wavelengths of the PerCP conjugate (bs-11636R-PerCP)?
PerCP on bs-11636R-PerCP: excitation / emission 482nm/677nm.