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Defensin beta 1 Polyclonal Antibody, RBITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-1163R-RBITC-100uL
Product Name Defensin beta 1 Polyclonal Antibody, RBITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from rat Defensin Beta 1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 83687
Swiss Prot O89117
Subcellular location Secreted
Synonyms Beta-defensin 1; BD-1; rBD-1; Defensin, beta 1; Defb1
Background Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Defensin beta 1 Polyclonal Antibody, RBITC Conjugated (bs-1163R-RBITC) validated for, and at what dilution?

bs-1163R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Defensin beta 1 Polyclonal Antibody, RBITC Conjugated (bs-1163R-RBITC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-1163R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Defensin beta 1 Polyclonal Antibody, RBITC Conjugated (bs-1163R-RBITC)?

KLH conjugated synthetic peptide derived from rat Defensin Beta 1

What are the excitation and emission wavelengths of the RBITC conjugate (bs-1163R-RBITC)?

RBITC on bs-1163R-RBITC: excitation / emission 540nm/625nm.