ALS2CR1 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11649R-BF594-100uL |
| Product Name | ALS2CR1 Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ALS2CR1/NIF3L1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | ALS2CR1; Amyotrophic lateral sclerosis 2 juvenile chromosome region, candidate 1; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 1 protein; CALS-7; MDS015; Ngg1 interacting factor 3-like 1; N3 Ngg1 interacting factor 3, S.pombe homolog-like 1; N3 NGG1 interacting factor 3-like 1 S. pombe; N3 NGG1 interacting factor 3-like 1; N3-like protein 1; N3L_HUMAN; N3L1. |
| Background | The NGG1 interacting factor 3-like 1 (NIF3L1) is a 377 amino acid protein expressed mainly in the cytoplasm of cells in several different tissues. It has been highly conserved throughout evolution, from bacteria to mammals. NIF3L participates in retinoic acid-primed neural differentiation of P19 embryonic carcinoma cells by cooperating with Trip15/CSN2, a transcriptional corepressor/component of COP9 signalosome. NIF3L1 interacts with itself and with the NIF3L1 binding protein 1 (NIF3L1 BP1), which is a novel protein presumed to contain a leucine zipper domain. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ALS2CR1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11649R-BF594) validated for, and at what dilution?
bs-11649R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11649R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ALS2CR1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11649R-BF594)?
KLH conjugated synthetic peptide derived from human ALS2CR1/NIF3L1
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-11649R-BF594)?
AbBy Fluor-594 on bs-11649R-BF594: excitation / emission 590nm/617nm.