COQ10B Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11656R-BF594-100uL |
| Product Name | COQ10B Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Cross-reactivity with COQ10A (91% homology with the immunogen sequence), |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Orangutan |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human COQ10B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 80219 |
| Swiss Prot | Q9H8M1 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Coenzyme Q10; Coenzyme Q10 B; Coenzyme Q10 homolog B; Coenzyme Q10B; CQ10B_HUMAN. |
| Background | COQ10B, Coenzyme Q10, is a 247 amino acid protein encoded by the human gene COQ10B. COQ10B is a mitochondrial protein that belongs to the COQ10 family. COQ10B is an essential biological cofactor which increases brain mitochondrial concentration and exerts neuroprotective effects. Plasma COQ10B levels decrease in patients with advanced chronic heart failure (CHF) while COQ10B levels in hyperthyroid patients are found among the lowest detected in human diseases. Likewise, COQ10B is elevated in hypothyroid subjects, also in subclinical conditions, suggesting the usefulness of this index in assessing metabolic status in thyroid disorders. It is believed that secretion of adrenal hormones is in some way related to COQ10B levels, both in augmented and reduced conditions. However, since thyroid hormones have an important role in modulating COQ10B levels and metabolism, when coexistent, thyroid deficiency seems to play a prevalent role in comparison with adrenal deficiency. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is COQ10B Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11656R-BF594) validated for, and at what dilution?
bs-11656R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does COQ10B Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11656R-BF594) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Orangutan.
How should bs-11656R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise COQ10B Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11656R-BF594)?
KLH conjugated synthetic peptide derived from human COQ10B
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-11656R-BF594)?
AbBy Fluor-594 on bs-11656R-BF594: excitation / emission 590nm/617nm.