PET112L Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11667R-PE-100uL |
| Product Name | PET112L Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PET112L |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | Cytochrome oxidase assembly factor PET112 homolog; GATB_HUMAN; GLU-ADT subunit B; HSPC199; mitochondrial; PET112; PET112 homolog yeast; PET112-like; Probable glutamyl-tRNAGln amidotransferase subunit B. |
| Background | PET112L is the human homolog of the S. cerevisiae COX assembly protein pet112, a protein that is believed to play an important role in the translation of mitochondrial genes. PET112L, also known as HSPC199 or Glu-ADT subunit B (glutamyl-tRNA(Gln) amidotransferase subunit B), is a 557 amino acid protein belonging to the gatB/gatE family of proteins (GatB subfamily) and is believed to play a role in energy metabolism. Localizing to mitochondria, PET112L is expressed in tissues such as heart and muscle, which exhibit high rates of oxidative phosphorylation. The gene encoding PET112L is overexpressed in recurrent ependymoma. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is PET112L Polyclonal Antibody, PE Conjugated (bs-11667R-PE) validated for, and at what dilution?
bs-11667R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PET112L Polyclonal Antibody, PE Conjugated (bs-11667R-PE) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow.
How should bs-11667R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PET112L Polyclonal Antibody, PE Conjugated (bs-11667R-PE)?
KLH conjugated synthetic peptide derived from human PET112L
What are the excitation and emission wavelengths of the PE conjugate (bs-11667R-PE)?
PE on bs-11667R-PE: excitation / emission 496,564nm/578nm.