THOP1 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11683R-BF750-100uL |
| Product Name | THOP1 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Thimet Oligopeptidase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | Endopeptidase 24.15; EP24.15; MEPD_HUMAN; MP78; Thimet oligopeptidase 1; Thimet oligopeptidase; Thop1; THOP1_HUMAN; EC 3.4.24.15. |
| Background | Thimet oligopeptidase, also designated soluble metallo-endopeptidase, is a cytoplasmic protein belonging to the peptidase M3 family. The gene for the protein maps against chromosome 19q13.3. Thimet oligopeptidase can degrade the b-Amyloid precursor protein and generate amyloidogenic fragments. It is important in cytoplasmic peptide degradation and involved in metabolism of neuropeptides that are less than 20 amino acids in length. Thimet oligopeptidase is highly expressed in testis but can also be detected in liver, lung and kidney. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is THOP1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11683R-BF750) validated for, and at what dilution?
bs-11683R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11683R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise THOP1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11683R-BF750)?
KLH conjugated synthetic peptide derived from human Thimet Oligopeptidase
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-11683R-BF750)?
AbBy Fluor-750 on bs-11683R-BF750: excitation / emission 749nm/775nm.