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THOP1 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Horse
Overview
Catalog # bs-11683R-PerCP-100uL
Product Name THOP1 Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Horse
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Thimet Oligopeptidase
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms Endopeptidase 24.15; EP24.15; MEPD_HUMAN; MP78; Thimet oligopeptidase 1; Thimet oligopeptidase; Thop1; THOP1_HUMAN; EC 3.4.24.15.
Background Thimet oligopeptidase, also designated soluble metallo-endopeptidase, is a cytoplasmic protein belonging to the peptidase M3 family. The gene for the protein maps against chromosome 19q13.3. Thimet oligopeptidase can degrade the b-Amyloid precursor protein and generate amyloidogenic fragments. It is important in cytoplasmic peptide degradation and involved in metabolism of neuropeptides that are less than 20 amino acids in length. Thimet oligopeptidase is highly expressed in testis but can also be detected in liver, lung and kidney.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is THOP1 Polyclonal Antibody, PerCP Conjugated (bs-11683R-PerCP) validated for, and at what dilution?

bs-11683R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-11683R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise THOP1 Polyclonal Antibody, PerCP Conjugated (bs-11683R-PerCP)?

KLH conjugated synthetic peptide derived from human Thimet Oligopeptidase

What are the excitation and emission wavelengths of the PerCP conjugate (bs-11683R-PerCP)?

PerCP on bs-11683R-PerCP: excitation / emission 482nm/677nm.