HIPPI Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11697R-PE-100uL |
| Product Name | HIPPI Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HIPPI |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | Dermal papilla derived protein 8; Dermal papilla-derived protein 8; DERP 8; DERP8; Esrrbl 1; Esrrbl1; ESRRBL1 protein; Estrogen related receptor beta like 1; Estrogen related receptor beta like protein 1; Estrogen-related receptor beta like 1; Estrogen-related receptor beta-like protein 1; FLJ10147; Hip1 Interacting Protein; HIP1 interacting protein HIPPI; Hip1 protein interactor; HIP1-interacting protein; HIPPI; Huntingtin interacting protein 1 interacting protein; Huntingtin interacting protein 1 protein interactor; Huntingtin-interacting protein-1 protein interactor; T 57; t57; T57_HUMAN; Intraflagellar transport 57 homolog Chlamydomonas; Intraflagellar transport 57 homolog; Intraflagellar transport protein 57 homolog; MHS4R2; Vestrogen related receptor beta like 1; Vestrogen-related receptor beta like 1; CDNA FLJ10147 fis clone HEMBA1003369. |
| Background | Programmed neuronal cell death is a feature of neurodegenerative disorders such as Alzheimer's and Huntington's disease, which occur later in human life. Huntington?s disease at the molecular and cell level is characterized by polyglutamine expansion of the protein huntingtin (Htt) that leads to apoptotis-mediated neurodegenerative loss of medium spiny neurons throughout the striatum. Polyglutamine expansion reduces the level of association between Hip-1 and Htt, thereby increasing levels of free Hip-1 that then can be the candidate protein Hippi (Hip-1 protein interactor). The Hippi-Hip-1 heterodimer is a pro-apoptotic complex that recruits procaspase-8 and initiates caspase-8 activation, which may contribute to the neuronal cell death observed in individuals diagnosed with Huntington?s disease. The human hippi gene maps to chromosome 3q13.13 and encodes a 429 amino acid protein. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is HIPPI Polyclonal Antibody, PE Conjugated (bs-11697R-PE) validated for, and at what dilution?
bs-11697R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIPPI Polyclonal Antibody, PE Conjugated (bs-11697R-PE) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Horse, Rabbit.
How should bs-11697R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIPPI Polyclonal Antibody, PE Conjugated (bs-11697R-PE)?
KLH conjugated synthetic peptide derived from human HIPPI
What are the excitation and emission wavelengths of the PE conjugate (bs-11697R-PE)?
PE on bs-11697R-PE: excitation / emission 496,564nm/578nm.