GDAP1 Polyclonal Antibody, PerCP-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11725r-percp-cy7-100uL |
| Product Name | GDAP1 Polyclonal Antibody, PerCP-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Orangutan |
| Specifications | |
| Conjugation | PerCP-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GDAP1 |
| Immunogen Range | 151-230/358 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Ganglioside induced dferentiation associated protein 1; Ganglioside-induced dferentiation-associated protein 1; GDAP1; GDAP1_HUMAN. |
| Background | Glutathione S-transferases (GSTs) function to conjugate reduced glutathione to many exogenous and endogenous hydrophobic electrophiles. Although it shares the carboxy and amino-terminal glutathione S-transferase domains, GDAP1 is characterized as a GST-like protein because it contains an extended GST domain II and a predicted transmembrane domain, two characteristics which are unusual for GST family members. GDAP1 may function in a signal transduction pathway that is responsible for ganglioside-induced neurite differentiation and also may play a role in protecting myelin membranes from free-radical damage. Mutations in the gene encoding GDAP1 is the cause of many forms of Charcot-Marie-Tooth disease, a common inherited disorder of the peripheral nervous system that is characterized by reduced nerve conduction velocities, slow progressive distal muscle atrophy and absent deep tendon reflexes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GDAP1 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-11725R-PerCP-Cy7) validated for, and at what dilution?
bs-11725R-PerCP-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GDAP1 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-11725R-PerCP-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Orangutan.
How should bs-11725R-PerCP-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GDAP1 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-11725R-PerCP-Cy7)?
KLH conjugated synthetic peptide derived from human GDAP1 Immunogen range: 151-230/358.
What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bs-11725R-PerCP-Cy7)?
PerCP-Cy7 on bs-11725R-PerCP-Cy7: excitation / emission 482nm/780nm.