N-cadherin Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1172R-PE-Cy5.5 |
| Product Name | N-cadherin Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human N-cadherin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1000 |
| Swiss Prot | P19022 |
| Subcellular location | Cell membrane |
| Synonyms | CDHN; NCAD; CD325; CDw325; Cadherin-2; Neural cadherin; N-cadherin; CDH2; CADH2_HUMAN |
| Background | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is N-cadherin Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-1172R-PE-CY5-5) validated for, and at what dilution?
BS-1172R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does N-cadherin Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-1172R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Horse, Rabbit.
How should BS-1172R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise N-cadherin Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-1172R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human N-cadherin
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-1172R-PE-CY5-5)?
PE-Cy5.5 on BS-1172R-PE-CY5-5: excitation / emission 488nm/694nm.