Engrailed 1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11744R-RBITC-100uL |
| Product Name | Engrailed 1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EN1/Engrailed 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2019 |
| Swiss Prot | Q05925 |
| Subcellular location | Nucleus |
| Synonyms | En 1; EN1; Engrailed 1; Engrailed; Engrailed homeobox 1; Engrailed homolog 1; HME1_HUMAN; Homeobox protein en-1; Homeobox protein engrailed 1; Homeobox protein engrailed-1; Hu-En-1; Mo-en.1. |
| Background | The engrailed-1 gene, EN1, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation. In Drosophila, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homo-logs, EN-1 and EN-2, produce different developmental defects that frequently are lethal. EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum. EN-1 and EN-2 regulate expres-sion of a-synuclein, a gene that is genetically linked to Parkinson?s disease. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Engrailed 1 Polyclonal Antibody, RBITC Conjugated (bs-11744R-RBITC) validated for, and at what dilution?
bs-11744R-RBITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Engrailed 1 Polyclonal Antibody, RBITC Conjugated (bs-11744R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-11744R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Engrailed 1 Polyclonal Antibody, RBITC Conjugated (bs-11744R-RBITC)?
KLH conjugated synthetic peptide derived from human EN1/Engrailed 1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-11744R-RBITC)?
RBITC on bs-11744R-RBITC: excitation / emission 540nm/625nm.