SGSH Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11756R-PE-Cy5-100uL |
| Product Name | SGSH Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Sulphamidase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6448 |
| Swiss Prot | P51688 |
| Subcellular location | Cytoplasm |
| Synonyms | HSS; SFMD; MPS3A; N-sulphoglucosamine sulphohydrolase; Sulfoglucosamine sulfamidase; Sulphamidase; SGSH |
| Background | Sulfatases are enzymes that hydrolyse a diverse range of sulfate esters. Deficiency of lysosomal sulfatases leads to human diseases characterized by the accumulation of either GAGs (glycosaminoglycans) or sulfolipids. Sulfamidase, also known as HSS, SFMD, MPS3A or SGSH, is a 502 amino acid lysosome that belongs to the sulfatase family. It has been suggested that sulfamidase may be involved in the lysosomal degradation of heparan sulfate. Defects in the gene encoding sulfamidase are the cause of Sanfilippo syndrome A, an autosomal recessive lysosomal storage disease caused by impaired degradation of heparan sulfate. Sanfilippo syndrome A is characterized by severe central nervous system degeneration but relatively mild somatic manifestations. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is SGSH Polyclonal Antibody, PE-Cy5 Conjugated (bs-11756R-PE-Cy5) validated for, and at what dilution?
bs-11756R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SGSH Polyclonal Antibody, PE-Cy5 Conjugated (bs-11756R-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog.
How should bs-11756R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SGSH Polyclonal Antibody, PE-Cy5 Conjugated (bs-11756R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human Sulphamidase
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-11756R-PE-Cy5)?
PE-Cy5 on bs-11756R-PE-Cy5: excitation / emission 488nm/670nm.