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OPA1 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-11764R-PerCP-100uL
Product Name OPA1 Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human OPA1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 84692
Swiss Prot Q8NEL0
Subcellular location Cytoplasm, Cell membrane
Synonyms SP17; NYD-SP17; Coiled-coil domain-containing protein 54; Testis development protein NYD-SP17; CCDC54
Background OPA1 is a 120kDa protein belonging to the dynamin family. The OPA1 gene has been localized to 3q29. The gene is targeted to mitochondria and is involved in mitochondrial biogenesis. Defects in OPA1 are a cause of optic atrophy type 1. OPA1 is mostly expressed in retina but can also be expressed in brain, testis, heart and skeletal muscle.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is OPA1 Polyclonal Antibody, PerCP Conjugated (bs-11764R-PerCP) validated for, and at what dilution?

bs-11764R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does OPA1 Polyclonal Antibody, PerCP Conjugated (bs-11764R-PerCP) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-11764R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise OPA1 Polyclonal Antibody, PerCP Conjugated (bs-11764R-PerCP)?

KLH conjugated synthetic peptide derived from human OPA1

What are the excitation and emission wavelengths of the PerCP conjugate (bs-11764R-PerCP)?

PerCP on bs-11764R-PerCP: excitation / emission 482nm/677nm.

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