Tmem18 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11799R-Cy5.5-100uL |
| Product Name | Tmem18 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Tmem18 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | DKFZp434C1714; TMEM18; TMM18_HUMAN; Transmembrane protein 18. |
| Background | TMEM18 is a 140 amino acid multi-pass membrane protein that localizes to the nuclear membrane and is expressed in the brain. TMEM18 functions as a cell migration modulator which enhances the glioma-specific migration ability of neural precursor and neural stem cells.Overexpression of TMEM18 increases migration of human and murine neural stem cells, whereas knockdown of TMEM18 mRNA reduces cellular migration. Two specific single nucleotide polymorphisms (SNPs) within the TMEM18 gene locus known as rs6548238 and rs756131 have been linked to obesity susceptibility. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Tmem18 Polyclonal Antibody, Cy5.5 Conjugated (BS-11799R-CY5-5) validated for, and at what dilution?
BS-11799R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-11799R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Tmem18 Polyclonal Antibody, Cy5.5 Conjugated (BS-11799R-CY5-5)?
KLH conjugated synthetic peptide derived from human Tmem18
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-11799R-CY5-5)?
Cy5.5 on BS-11799R-CY5-5: excitation / emission 675nm/694nm.