RhoA/C Polyclonal Antibody, Cy7 Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-1180R-Cy7
Product Name RhoA/C Polyclonal Antibody, Cy7 Conjugated
Applications FCM, IF(IHC-P), IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RhoA
Immunogen Range 90-140/193
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Target
Gene ID 387, 389
Swiss Prot P61586
Subcellular location Cytoplasm, Helical
Synonyms ARHA; ARH12; RHO12; RHOH12; Transforming protein RhoA; Rho cDNA clone 12; h12; RHOA; Rho-related GTP-binding protein RhoC; Rho cDNA clone 9; ARH9, ARHC.
Background Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200