CBLN4 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11816R-BF594-100uL |
| Product Name | CBLN4 Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CBLN4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | CBLN 4; CBLNL1; Cerebellin 4; Cerebellin 4 precursor; Cerebellin like glycoprotein 1; Cerebellin precursor like 1; Cerebellin4; CBLN4_HUMAN. |
| Background | Cerebellin (CER), which was originally isolated from rat cerebellum, is a hexadecapeptide derived from a larger precursor called Cerebellin 1, also designated precerebellin 1 or Cbln1. Four propeptides, Cerebellin 1, Cerebellin 2 (Cbln2), Cerebellin 3 (Cbln3) and Cerebellin 4 (Cbln4), comprise the precerebellin subfamily within the C1q protein family. Cerebellin family members act as transneuronal regulators of synapse development and synaptic plasticity in various brain regions. Cerebellin and its metabolite, des-Ser(1)Cer, are also expressed in several extra-cerebellar tissues, including adrenal gland. Cerebellin 1, 2 and 3 assemble into homomeric and heteromeric complexes, thereby influencing each other?s degradation and secretion. Cerebellin 3 is not able to form homomeric complexes, and can only be secreted upon forming a heteromeric complex with Cerebellin 1. Decreased concentrations of Cerebellin have been found in the brain of patients with olivopontocerebellar atrophy (OPCA) and Shy-Drager syndrome, suggesting a role for Cerebellin in the pathology of these diseases. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CBLN4 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11816R-BF594) validated for, and at what dilution?
bs-11816R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11816R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CBLN4 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11816R-BF594)?
KLH conjugated synthetic peptide derived from human CBLN4
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-11816R-BF594)?
AbBy Fluor-594 on bs-11816R-BF594: excitation / emission 590nm/617nm.