CA10 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11830R-BF750-100uL |
| Product Name | CA10 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Carbonic anhydrase X |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Extracellular matrix |
| Synonyms | CA 10; CA-RP X; CA10; CAH10_HUMAN; Carbonic anhydrase related protein; Carbonic anhydrase-related protein 10; Carbonic anhydrase-related protein X; CARP X; CARPX; Cerebral protein 15; HUCEP 15. |
| Background | Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes responsible for catalyzing the reversible hydration of carbon dioxide. CAs show extensive diversity in their distribution and subcellular localization. They are involved in a variety of biological processes, including calcification, bone resorption, respiration, acid-base balance and the formation of aqueous humor, saliva, gastric juice and cerebrospinal fluid. CA X also referred to as Carbonic anhydrase-related protein X (CA-RP X) or Cerebral protein 15, is a member of the carbonic anhydrase family that lacks two of the three Zn-binding motifs essential for carbonic anhydrase activity. For this reason, CA X does not exhibit catalytic activity. It is expressed primarily in brain and kidney and may play a role in brain development. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CA10 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11830R-BF750) validated for, and at what dilution?
bs-11830R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11830R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CA10 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11830R-BF750)?
KLH conjugated synthetic peptide derived from human Carbonic anhydrase X
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-11830R-BF750)?
AbBy Fluor-750 on bs-11830R-BF750: excitation / emission 749nm/775nm.