DACT3 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11837R-BF594 |
| Product Name | DACT3 Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DACT3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | Antagonist of beta-catenin Dapper homolog 3; Arginine rich region 1; Arginine rich region 1 protein; Arginine-rich region 1 protein; Dact3 antibody; DACT3_HUMAN; Dapper antagonist of beta catenin 3; Dapper antagonist of beta catenin homolog 3 Xenopus laevis; Dapper homolog 3; Dapper3; Dpr3 antibodyRRR1; RRR1; Thymus expressed gene 3 like. |
| Background | Dapper3 is a 629 amino acid protein and mammalian homolog of the Xenopus laevis protein dapper. As a member of the dapper family, Dapper3 plays a role in postnatal brain development and contains a C-terminal PDZ-binding motif that facilitates interaction with the PDZ domains of DSH (Dishevelled) family proteins. As the predominant dapper family member found in adult brain, Dapper 3 localizes to hippocampus, Purkinje cell layer and every layer of the dorsal forebrain and cerebral cortex and is also found in the developing murine central nervous system. Dapper3 is also expressed in uterus, ventral somites, branchial arch mesenchyme, aortic sac, aortic arches, limb bud mesenchyme and craniofacical mesenchyme. Mapping to human chromosome 19q13.32, Dapper3 has been identified as a negative regulator of Wnt/beta-catenin signaling in colorectal cancer. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DACT3 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11837R-BF594) validated for, and at what dilution?
bs-11837R-BF594 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11837R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DACT3 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11837R-BF594)?
KLH conjugated synthetic peptide derived from human DACT3
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-11837R-BF594)?
AbBy Fluor-594 on bs-11837R-BF594: excitation / emission 590nm/617nm.