DNTNP Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11839R-BF750 |
| Product Name | DNTNP Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Dog |
| Predicted Reactivity | Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DNTNP/FAM53A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | DNTNP; Dorsal neural-tube nuclear protein; FA53A_HUMAN; FAM53A; family with sequence similarity 53, member A; Protein FAM53A. |
| Background | Dorsal neural-tube nuclear protein is a 398 amino acid nuclear protein. DNTNP is thought to play an important role in neural development by specifying dorsal cell fates within the neural tube. Widely expressed in the dorsal neural tube, DNTNP is most highly expressed in the dorsal regions of the midbrain, the hindbrain, the diencephalon, and the spinal neural tube and is expressed at lower levels in the branchial arches, the telencephalon, the heart, and somites of developing embryos. DNTNP is encoded by a gene located on chromosome 4 which contains many genes including the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington's disease. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DNTNP Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11839R-BF750) validated for, and at what dilution?
bs-11839R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DNTNP Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11839R-BF750) react with?
Tested reactivity: Human, Dog. Predicted reactivity (expected from sequence homology but not yet tested): Chicken.
How should bs-11839R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DNTNP Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11839R-BF750)?
KLH conjugated synthetic peptide derived from human DNTNP/FAM53A
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-11839R-BF750)?
AbBy Fluor-750 on bs-11839R-BF750: excitation / emission 749nm/775nm.