KALRN Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11861R-BF488 |
| Product Name | KALRN Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KALRN/Duo |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | Duo; FLJ16443; HAPIP; Huntingtin associated protein interacting protein duo ; Huntingtin-associated protein-interacting protein; Kalirin isoform 2; Kalirin; KALRN ; KALRN_HUMAN ; Protein Duo; RhoGEF kinase; Serine/threonine kinase with Dbl and pleckstrin homology domains ; Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain; TRAD. |
| Background | HAP1 (huntingtin-associated protein 1) binds to huntingtin (1). Huntingtin is a protein that contains a polyglutamine region and when the number of glutamine repeats exceeds 35, the gene encodes a version of huntingtin that leads to Huntington?s disease (HD) (2,3). The ability of HAP1 to bind to huntingtin is enhanced by an expanded polyglutamine repeat region (1). HAP1 shows neuronal localization and moves with huntingtin in nerve fibers (4,5). HAP1 is primarily expressed in brain tissue, with greater expression in the olfactory bulb and brain stem (1). Mouse HAP1 is localized to membrane-bound organelles including large endosomes, tubulovesicular structures and budding vesicles in neurons (6). Duo, also designated huntingtin-associated protein interacting protein or HAPIP, binds Huntingtin-associated protein 1 (HAP1) and may have a role in vesicle trafficking and cytoskeletal function. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is KALRN Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-11861R-BF488) validated for, and at what dilution?
bs-11861R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11861R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KALRN Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-11861R-BF488)?
KLH conjugated synthetic peptide derived from human KALRN/Duo
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-11861R-BF488)?
AbBy Fluor-488 on bs-11861R-BF488: excitation / emission 499nm/519nm.