KIRREL3 Polyclonal Antibody, PE-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11864R-PE-Cy7 |
| Product Name | KIRREL3 Polyclonal Antibody, PE-Cy7 Conjugated |
| Applications |
FCM
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Chicken |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KIRREL3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | Kin of IRRE like 3 Drosophila; Kin of IRRE like 3; Kin of irregular chiasm like protein 3 ; KIRRE; KIRREL 3; KIRREL-3; MRD4; NEPH2; Nephrin like 2; PRO19814; PRO4502; yUNQ5923; KIRR3_HUMAN. |
| Background | NEPH2 is a 778 amino acid single-pass type I membrane protein that belongs to the nephrin-like protein family and immunoglobulin superfamily. Expressed in both fetal and adult brain, as well as podocytes of kidney glomeruli, NEPH2 contains five Ig-like C2-type (immunoglobulin-like) domains and is thought to plaly a role in the hematopoetic supportive capacity of stroma cells. NEPH2 undergoes alternative splicing to produce two isoforms and contains a C-terminal cytoplasmic domain which it uses to interact with Podocin, a podocyte protein involved in ultrafiltration. Defects in the gene encoding NEPH2 are associated with mental retardation autosomal dominant type 4 (MRD4). |
| Application Dilution | |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is KIRREL3 Polyclonal Antibody, PE-Cy7 Conjugated (bs-11864R-PE-Cy7) validated for, and at what dilution?
bs-11864R-PE-Cy7 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KIRREL3 Polyclonal Antibody, PE-Cy7 Conjugated (bs-11864R-PE-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Chicken.
How should bs-11864R-PE-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KIRREL3 Polyclonal Antibody, PE-Cy7 Conjugated (bs-11864R-PE-Cy7)?
KLH conjugated synthetic peptide derived from human KIRREL3
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-11864R-PE-Cy7)?
PE-Cy7 on bs-11864R-PE-Cy7: excitation / emission 488nm/780nm.