LRRTM2 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11877R-Cy3-100uL |
| Product Name | LRRTM2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LRRTM2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | KIAA0416; Leucine rich repeat transmembrane neuronal 2; Leucine rich repeat transmembrane neuronal 2 protein; Leucine rich repeat transmembrane protein 2; Leucine-rich repeat neuronal 2 protein; Leucine-rich repeat transmembrane neuronal protein 2; LRRN2; LRRT2_HUMAN; Lrrtm2. |
| Background | The leucine-rich (LRR) repeat is a 20-30 amino acid motif that forms a hydrophobic Alpha/Beta horseshoe fold, allowing it to accommodate several leucine residues within a tightly packed core. All LRR repeats contain a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif. The LRRTM protein family plays a role in the regulation of various cellular events during nervous system development. Localizing predominantly to the nervous system, LRRTM family members are known to exhibit synaptogenic activity. LRRTM2 (leucine rich repeat transmembrane neuronal 2), also known as LRRN2, is a 516 amino acid single-pass type I membrane protein involved in the development maintenance of the vertebrate nervous system. Expressed in kidney and neuronal tissues, LRRTM2 contains ten LRR repeats and belongs to the LRRTM family. LRRTM2 is encoded by a gene that maps to human chromosome 5q31.2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is LRRTM2 Polyclonal Antibody, Cy3 Conjugated (bs-11877R-Cy3) validated for, and at what dilution?
bs-11877R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LRRTM2 Polyclonal Antibody, Cy3 Conjugated (bs-11877R-Cy3) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Rabbit.
How should bs-11877R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LRRTM2 Polyclonal Antibody, Cy3 Conjugated (bs-11877R-Cy3)?
KLH conjugated synthetic peptide derived from human LRRTM2
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-11877R-Cy3)?
Cy3 on bs-11877R-Cy3: excitation / emission 512,550nm/570,615nm.