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FRA2 Polyclonal Antibody, RBITC Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-1188R-RBITC
Product Name FRA2 Polyclonal Antibody, RBITC Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody has around 75% homology with FRA1.
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Chicken, Rabbit, GuineaPig
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FRA2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2355
Swiss Prot P15408
Subcellular location Nucleus
Synonyms FRA2; Fos-related antigen 2; FRA-2; FOSL2
Background Controls osteoclast survival and size. As a dimer with JUN, activates LIF transcription. Activates CEBPB transcription in PGE2-activated osteoblasts.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is FRA2 Polyclonal Antibody, RBITC Conjugated (bs-1188R-RBITC) validated for, and at what dilution?

bs-1188R-RBITC is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FRA2 Polyclonal Antibody, RBITC Conjugated (bs-1188R-RBITC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Chicken, Rabbit, GuineaPig.

How should bs-1188R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FRA2 Polyclonal Antibody, RBITC Conjugated (bs-1188R-RBITC)?

KLH conjugated synthetic peptide derived from human FRA2

What are the excitation and emission wavelengths of the RBITC conjugate (bs-1188R-RBITC)?

RBITC on bs-1188R-RBITC: excitation / emission 540nm/625nm.