NELL2 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11895R-BF350 |
| Product Name | NELL2 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NELL2/NRP2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted |
| Synonyms | A330108N19Rik; Mel91; MEL91 protein; NEL like 2 chicken; NEL-like protein 2; Nel-related protein 2; NELL2; NELL2_HUMAN; NRP2; Protein kinase C-binding protein NELL2; R75516. |
| Background | NELL2 is a secreted glycoprotein with one N-terminal TSP-like domain, five VWFC (von Willebrand factor C) domains and six EGF-like repeats that participate in calcium binding. NELL2 exists as a homotrimer associated with the endoplasmic reticulum (ER) and is specifically expressed in neurons playing a role in calcium-dependent intracellular events. NELL2 may act as a trophic factor in addition to its role as a signaling molecule implicated in the regulation of cell growth and differentiation. Due to alternative splicing, an additional NELL2 isoform exists that is identical to full length NELL2 except that it lacks the signal peptide that directs secretion. The additional isoform is therefore retained in the cytosol and functions as a non-secreted cytoplasmic protein that interacts with PKC bI. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NELL2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11895R-BF350) validated for, and at what dilution?
bs-11895R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NELL2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11895R-BF350) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse.
How should bs-11895R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NELL2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11895R-BF350)?
KLH conjugated synthetic peptide derived from human NELL2/NRP2
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-11895R-BF350)?
AbBy Fluor-350 on bs-11895R-BF350: excitation / emission 346nm/442nm.