NFIX Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11900R-BF750 |
| Product Name | NFIX Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NFIX |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | CCAAT box binding transcription factor; CCAAT-box-binding transcription factor; CTF; NF-I/X; NF1-X; NF1A; NF1X; NFI X; NFI-X; NFI/X; NFIX; NFIX_HUMAN; Nuclear factor 1 X type; Nuclear factor 1 X-type; Nuclear factor 1/X; Nuclear factor I/X; TGGCA binding protein; TGGCA-binding protein. |
| Background | Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NFIX Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11900R-BF750) validated for, and at what dilution?
bs-11900R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NFIX Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11900R-BF750) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse.
How should bs-11900R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NFIX Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11900R-BF750)?
KLH conjugated synthetic peptide derived from human NFIX
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-11900R-BF750)?
AbBy Fluor-750 on bs-11900R-BF750: excitation / emission 749nm/775nm.