NHLH1 Polyclonal Antibody, APC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11901R-APC |
| Product Name | NHLH1 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NHLH1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | bHLHa35; Class A basic helix-loop-helix protein 35; Helix-loop-helix protein 1; HEN-1; HEN1; HEN1_HUMAN; Nescient helix loop helix 1; Nhlh1; NSCL; NSCL-1; NSCL1; OTTHUMP00000031860. |
| Background | The helix-loop-helix (HLH) structures are known motifs commonly found in membrane-active and DNA-binding proteins. The helix-loop-helix proteins HEN1 and HEN2 are DNA-binding proteins that may be involved in cell-type determination in the early nervous system. Studies of expression in normal tissues have demonstrated expression of NHLH1/NSCL-1 and NHLH2/NSCL-2, the genes encoding HEN1 and HEN2, in the developing central and peripheral nervous system, specifically in developing neurons. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NHLH1 Polyclonal Antibody, APC Conjugated (bs-11901R-APC) validated for, and at what dilution?
bs-11901R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11901R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NHLH1 Polyclonal Antibody, APC Conjugated (bs-11901R-APC)?
KLH conjugated synthetic peptide derived from human NHLH1
What are the excitation and emission wavelengths of the APC conjugate (bs-11901R-APC)?
APC on bs-11901R-APC: excitation / emission 650nm/660nm.