NME4 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11902R-PerCP-Cy5.5-100uL |
| Product Name | NME4 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NME4/nm23-H4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | mitochondrial; NDK; NDKM_HUMAN; NDP kinase; NDP kinase, mitochondrial; NDPKD; nm23 H4; nm23-H4; NM23D; Nm23M4; NME4; Non metastatic cells 4 protein expressed in; Nucleoside diphosphate kinase D; Nucleoside diphosphate kinase, mitochondrial. |
| Background | The nm23 gene (Metastasis inhibition factor nm23), a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. Based on sequence analysis, nm23, also designated nucleoside diphosphate kinase A (NDK A) or Tumor metastatic process-associated protein, appears to be highly related to nucleotide diphosphate kinases (NDP). NDP kinases A and B are identical to two isotypes of human nm23 homologs, nm23-H1 and nm23-H2, respectively. nm23-H2 is also identical in sequence to PuF, a transcription factor that binds to nuclease-hypersensitive elements at positions 142 to 115 of the human c-Myc promoter. nm23-H3 and nm23-H4 are important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. nm23-H4 localizes to the mitochondrial intermembrane space and is widely expressed, with higher levels detected in prostate, heart, liver, small intestine, and skeletal muscle tissues. Low amounts of nm23-H4 are observed in the brain and in blood leukocytes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NME4 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-11902R-PERCP-CY5-5) validated for, and at what dilution?
BS-11902R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NME4 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-11902R-PERCP-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Pig, Rabbit.
How should BS-11902R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NME4 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-11902R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human NME4/nm23-H4
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-11902R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-11902R-PERCP-CY5-5: excitation / emission 482nm/694nm.