NOTUM Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11904R-BF350 |
| Product Name | NOTUM Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NOTUM |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 147111 |
| Swiss Prot | Q6P988 |
| Subcellular location | Secreted |
| Synonyms | NOTUM; notum pectinacetylesterase homolog Drosophila; Notum pectinacetylesterase homolog; NOTUM_HUMAN. |
| Background | Notum is a 496 amino acid secreted protein that belongs to the pectinacetylesterase family and may deacetylate GlcNAc residues on cell surface glycans. The gene that encodes Notum consists of approximately 9,334 bases and maps to human chromosome 17q25.3. Encoding more than 1,200 genes, chromosome 17 comprises over 2.5% of the human genome. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of ovary, colon, prostate gland and fallopian tubes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NOTUM Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11904R-BF350) validated for, and at what dilution?
bs-11904R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11904R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NOTUM Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11904R-BF350)?
KLH conjugated synthetic peptide derived from human NOTUM
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-11904R-BF350)?
AbBy Fluor-350 on bs-11904R-BF350: excitation / emission 346nm/442nm.