Collagen II Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11929R-BF647 |
| Product Name | Collagen II Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Collagen II |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | Collagen II alpha 1; COL2A1; COL2A1 protein; collagen, type II, alpha 1; collagen alpha-1II; type II collagen; alpha-1 type II collagen; alpha1 type II collagen; Col2a1; AOM; Cartilage collagen; Chondrocalcin; COL11A3; Collagen alpha 1II chain precursor; Collagen type II alpha 1 primary osteoarthritis spondyloepiphyseal dysplasia congenital; MGC131516; SEDC. |
| Background | Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate with specificities to collagens. The development of type specific is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Collagen II Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-11929R-BF647) validated for, and at what dilution?
bs-11929R-BF647 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Collagen II Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-11929R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Chicken, Rabbit.
How should bs-11929R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Collagen II Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-11929R-BF647)?
KLH conjugated synthetic peptide derived from human Collagen II
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-11929R-BF647)?
AbBy Fluor-647 on bs-11929R-BF647: excitation / emission 650nm/665nm.