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SHISA9 Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Horse
  • Rabbit
Overview
Catalog # bs-11950R-APC
Product Name SHISA9 Polyclonal Antibody, APC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SHISA9
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cell membrane
Synonyms 2700045P11Rik; CKAMP44; Cystine knot AMPAR modulating protein; DKFZp686D24206; DKFZp686E24206; FLJ10015; FLJ37653; FLJ58277; FLJ99079; FLJ99234; Protein shisa-9; Shisa homolog 9 Xenopus laevis; Shisa homolog 9; shisa9; SHSA9_HUMAN.
Background Regulator of short-term neuronal synaptic plasticity in the dentate gyrus. Associates with AMPA receptors (ionotropic glutamate receptors) in synaptic spines and promotes AMPA receptor desensitization at excitatory synapses.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SHISA9 Polyclonal Antibody, APC Conjugated (bs-11950R-APC) validated for, and at what dilution?

bs-11950R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SHISA9 Polyclonal Antibody, APC Conjugated (bs-11950R-APC) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Horse, Rabbit.

How should bs-11950R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SHISA9 Polyclonal Antibody, APC Conjugated (bs-11950R-APC)?

KLH conjugated synthetic peptide derived from human SHISA9

What are the excitation and emission wavelengths of the APC conjugate (bs-11950R-APC)?

APC on bs-11950R-APC: excitation / emission 650nm/660nm.