SPOCK1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11965R-RBITC |
| Product Name | SPOCK1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SPOCK1/Testican 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | Sparc/osteonectin, cwcv and kazal like domains proteoglycan 1; SPOCK; Testican 1; Testican1; Testican-1;; TIC1; TICN1; TICN1_HUMAN. |
| Background | The Testican family, also designated the BM-40/SPARC/osteonectin family, is composed of highly conserved, extracellular, calcium-binding, sulfate proteoglycans. Expression of Testicans is detected in a variety of tissues, but is most abundant in brain. Family members include Testican-1, Testican-2, Testican-3 and an amino-terminal splice variant of Testican-3, designated N-Tes. Most Testicans inhibit MT-MMPs, thereby inhibiting the activity of pro-MMP-2. Testican-1 is specifically expressed in the thalamus of the brain, and is upregulated in activated astroglial cells of the cerebrum where it mediates neuronal attachment and matrix metalloproteinase activation. After a neural injury, such as a cerebral stroke, Testican-1 expression is upregulated in astrocyte cells in order to inhibit the ability of the protein Neuro-2a to form neurite extensions. Testican-1 is also a component of joint and of the growth plate cartilage that may participate in the regulation of matrix turnover. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is SPOCK1 Polyclonal Antibody, RBITC Conjugated (bs-11965R-RBITC) validated for, and at what dilution?
bs-11965R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SPOCK1 Polyclonal Antibody, RBITC Conjugated (bs-11965R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Sheep, Pig, Rabbit.
How should bs-11965R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SPOCK1 Polyclonal Antibody, RBITC Conjugated (bs-11965R-RBITC)?
KLH conjugated synthetic peptide derived from human SPOCK1/Testican 1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-11965R-RBITC)?
RBITC on bs-11965R-RBITC: excitation / emission 540nm/625nm.