NCS1 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11977R-BF488 |
| Product Name | NCS1 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NCS1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 9430075O15Rik; A730032G13Rik; AI836659; DKFZp761L1223; FLUP; Frequenin; Frequenin homolog Drosophila; Frequenin homolog; Frequenin like protein; Frequenin, Drosophila, homolog of; Frequenin-like protein; Frequenin-like ubiquitous protein; Mfreq; NCS 1; NCS-1; ncs1; NCS1_HUMAN; Neuronal calcium sensor 1; Neuronal Calicum Sensor 1. |
| Background | NCS-1 (for neuronal calcium sensor-1, also designated frequenin) belongs to a highly conserved family of EF-hand-containing Ca++-binding proteins expressed mainly in neurons. NCS-1 is localized to neuronal cell bodies and axons throughout the brain and spinal cord. It is also expressed in glial cells and in neuroendocrine bovine adrenal chromaffin and PC12 cells. NCS-1 is a regulatory protein involved in Ca++-dependent exocytosis of synaptic vesicles and dense core granules. NCS-1 also functions in the voltage-independent autocrine pathway that negatively regulates non-L-type Ca++ channels. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NCS1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-11977R-BF488) validated for, and at what dilution?
bs-11977R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NCS1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-11977R-BF488) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow.
How should bs-11977R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NCS1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-11977R-BF488)?
KLH conjugated synthetic peptide derived from human NCS1
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-11977R-BF488)?
AbBy Fluor-488 on bs-11977R-BF488: excitation / emission 499nm/519nm.