PMCA3/Calcium Pump PMCA3 ATPase Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PMCA3\/Calcium Pump PMCA3 ATPase Polyclonal Antibody, Unconjugated (bs-11985R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PMCA3\/Calcium Pump PMCA3 ATPase Polyclonal Antibody, Unconjugated (bs-11985R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PMCA3\/Calcium Pump PMCA3 ATPase Polyclonal Antibody, Unconjugated (bs-11985R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PMCA3\/Calcium Pump PMCA3 ATPase Polyclonal Antibody, Unconjugated (bs-11985R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PMCA3\/Calcium Pump PMCA3 ATPase Polyclonal Antibody, Unconjugated (bs-11985R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PMCA3\/Calcium Pump PMCA3 ATPase Polyclonal Antibody, Unconjugated (bs-11985R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IHC-F

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-11985R
Product Name	PMCA3/Calcium Pump PMCA3 ATPase Antibody
Applications	WB, IHC-P, IHC-F
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PMCA3/Calcium Pump PMCA3 ATPase
Immunogen Range	351-450/1220
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	490
Swiss Prot	P20020
Subcellular location	Cell membrane
Synonyms	ATP2B3; ATP2B3 protein; ATPase Ca++ transporting plasma membrane 3; Plasma membrane calcium ATPase 3; Plasma membrane calcium ATPase isoform 3; Plasma membrane calcium pump isoform 3; Plasma membrane calcium transporting ATPase 3; PMCA 3; PMCA3; PMCA3a; AT2B3_HUMAN.
Background	Plasma membrane-type Ca2+-ATPases (PMCAs) mediate the export of bivalent calcium ions from eukaryotic cells. As members of the P class of ion-motive ATPases, PMCAs are a functionally diverse group of proteins that are derived from alternatively spliced transcripts originating from four distinct genes, PMCA1, 2, 3, and 4. The expression of different PMCA isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, and with respect to the physiological needs of specific cell and tissue types. Spatial and temporal rates of resting intracellular Ca2+ concentrations and Ca2+ signaling in eukaryotic cells are dependent on the array of PMCA isoforms that are expressed in concert with the rate of Ca2+ export. PMCA3 expression is confined to brain and skeletal muscle. The PMCA4 gene is located on human chromosome 1q25 and is ubiquitously expressed.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500

Applications

Reactivity

Predicted Reactivity

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