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Phospho-Serine Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Chicken
  • Monkey
  • Goose
Overview
Catalog # bs-11993R-PE-100uL
Product Name Phospho-Serine Polyclonal Antibody, PE Conjugated
Applications
WB IF
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Chicken, Monkey, Goose
Specifications
Conjugation PE
Host Rabbit
Source Phosphoserine-KLH
Modification Site Phosphoserine
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms Ser; pS; p-S; pSER; p-SER.
Background Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
Application Dilution
WB 1:300-5000
IF

Frequently asked questions

Which applications is Phospho-Serine Polyclonal Antibody, PE Conjugated (bs-11993R-PE) validated for, and at what dilution?

bs-11993R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-Serine Polyclonal Antibody, PE Conjugated (bs-11993R-PE) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Chicken, Monkey, Goose.

How should bs-11993R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-Serine Polyclonal Antibody, PE Conjugated (bs-11993R-PE)?

Phosphoserine-KLH

What are the excitation and emission wavelengths of the PE conjugate (bs-11993R-PE)?

PE on bs-11993R-PE: excitation / emission 496,564nm/578nm.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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