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CHRM4 Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • FCM

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bs-11998R-PE-Cy5
Product Name CHRM4 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
FCM
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CHRM4/mAChR M4
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1132
Swiss Prot P08173
Subcellular location Cell membrane
Synonyms HM4; M4R; Muscarinic acetylcholine receptor M4; CHRM4
Background The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
Application Dilution
FCM 1:20-100

Frequently asked questions

Which applications is CHRM4 Polyclonal Antibody, PE-Cy5 Conjugated (bs-11998R-PE-Cy5) validated for, and at what dilution?

bs-11998R-PE-Cy5 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CHRM4 Polyclonal Antibody, PE-Cy5 Conjugated (bs-11998R-PE-Cy5) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bs-11998R-PE-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CHRM4 Polyclonal Antibody, PE-Cy5 Conjugated (bs-11998R-PE-Cy5)?

KLH conjugated synthetic peptide derived from human CHRM4/mAChR M4

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-11998R-PE-Cy5)?

PE-Cy5 on bs-11998R-PE-Cy5: excitation / emission 488nm/670nm.