AKAP7 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11999R-BF647 |
| Product Name | AKAP7 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AKAP7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9465 |
| Swiss Prot | O43687 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | AKAP15; AKAP18; A-kinase anchor protein 7 isoforms alpha and beta; AKAP-7 isoforms alpha and beta; A-kinase anchor protein 18 kDa; AKAP 18; Protein kinase A-anchoring protein 7 isoforms alpha/beta; PRKA7 isoforms alpha/beta; AKAP7 |
| Background | Targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is AKAP7 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-11999R-BF647) validated for, and at what dilution?
bs-11999R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AKAP7 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-11999R-BF647) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Sheep, Pig, Horse.
How should bs-11999R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AKAP7 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-11999R-BF647)?
KLH conjugated synthetic peptide derived from human AKAP7
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-11999R-BF647)?
AbBy Fluor-647 on bs-11999R-BF647: excitation / emission 650nm/665nm.