GRM1 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12011R-BF488 |
| Product Name | GRM1 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Zebrafish |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MGLUR1A/Metabotropic Glutamate Receptor 1a |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | G protein coupled receptor family C group 1 member A; Glutamate receptor metabotropic 1 alpha; Glutamate receptor metabotropic 1; GPRC1A; GRM 1; GRM1 alpha; GRM1; GRM1A; Human metabotropic glutamate receptor 1 alpha mRNA complete cds; Metabotropic glutamate receptor 1 alpha; Metabotropic glutamate receptor 1; mGlu1; MGLUR1; MGLUR1A; mGluR1alpha. |
| Background | L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternative splice variants of the GRM1 gene have been described but their full-length nature has not been determined. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GRM1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-12011R-BF488) validated for, and at what dilution?
bs-12011R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GRM1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-12011R-BF488) react with?
Tested reactivity: Human, Mouse, Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-12011R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GRM1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-12011R-BF488)?
KLH conjugated synthetic peptide derived from human MGLUR1A/Metabotropic Glutamate Receptor 1a
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-12011R-BF488)?
AbBy Fluor-488 on bs-12011R-BF488: excitation / emission 499nm/519nm.