GRM1 + GRM5 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12014R-BF647 |
| Product Name | GRM1 + GRM5 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MGLUR1 + MGLUR5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | MGLUR1+MGLUR5; GPRC1A; GPRC1E; GRM1; GRM1A; GRM5; Metabotropic glutamate receptor 1; Metabotropic glutamate receptor 5; mGlu1; mGlu5; MGLUR1; MGLUR1A; MGLUR5; MGLUR5B; GRM1_HUMAN; GRM5_HUMAN. |
| Background | Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainite receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins. These receptors are called metabotropic glutamate receptors. In expression systems, Group-I receptors stimulate phospholipase C as revealed by an increase in phosphoinositide turnover and calcium release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. The Group-I receptors include Metabotropic Glutamate Receptor 5 and Metabotropic Glutamate Receptor 1a. The Group-II receptors in include mGluR2 and mGluR3. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is GRM1 + GRM5 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12014R-BF647) validated for, and at what dilution?
bs-12014R-BF647 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GRM1 + GRM5 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12014R-BF647) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-12014R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GRM1 + GRM5 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12014R-BF647)?
KLH conjugated synthetic peptide derived from human MGLUR1 + MGLUR5
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-12014R-BF647)?
AbBy Fluor-647 on bs-12014R-BF647: excitation / emission 650nm/665nm.