GPR125 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12021R-HRP-100uL |
| Product Name | GPR125 Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GPR125 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | FLJ38547; G protein coupled receptor 125; PGR21; Probable G protein coupled receptor 125 precursor; TEM5 like; TEM5L; GP125_HUMAN; GPCR125. |
| Background | G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR125 (G protein-coupled receptor 125), also known as PGR21 or TEM5L, is a 1,321 amino acid multi-pass membrane protein belonging to the G-protein coupled receptor 2 family and the LN-TM7 subfamily. Considered a novel orphan adhesion-type G-protein-coupled receptor, GPR125 has five leucine rich repeats (LRR), an immunoglobulin (Ig) domain and a GPS domain. GPR125 may play a functional role in choroidal and hippocampal response to brain injury. It is also suggested that GPR125 may be a marker for spermatogonial stem cells. Four isoforms of GPR125 exists due to alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is GPR125 Polyclonal Antibody, HRP Conjugated (bs-12021R-HRP) validated for, and at what dilution?
bs-12021R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPR125 Polyclonal Antibody, HRP Conjugated (bs-12021R-HRP) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Horse.
How should bs-12021R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPR125 Polyclonal Antibody, HRP Conjugated (bs-12021R-HRP)?
KLH conjugated synthetic peptide derived from human GPR125