GPR105 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12028R-Cy3-100uL |
| Product Name | GPR105 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human G-protein coupled receptor 105 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | A330108O13Rik; G protein coupled receptor for UDP-glucose; G protein-coupled receptor 105; G protein-coupled receptor VTR 15-20; G-protein coupled receptor 105; GPR105; KIAA0001; P2RY14; P2Y purinoceptor 14; P2Y14 receptor; P2Y14; P2Y14 receptor; P2Y14_HUMAN; Purinergic receptor P2Y G protein coupled 14; Similar to TYPE-1 Angiotensin II receptor; UDP-glucose receptor; GPCR GPR105. |
| Background | G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation). GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane-spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR105, also designated P2Y14, is widely expressed throughout many brain regions where it localizes to glial cells, and specifically co-localizes with astrocytes. GPR105 is upregulated when a tissue is immunologically challenged with lipopolysaccharide, leading to the theory that GPR105 may play an important role in modulating peripheral and neuroimmune function. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GPR105 Polyclonal Antibody, Cy3 Conjugated (bs-12028R-Cy3) validated for, and at what dilution?
bs-12028R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12028R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPR105 Polyclonal Antibody, Cy3 Conjugated (bs-12028R-Cy3)?
KLH conjugated synthetic peptide derived from human G-protein coupled receptor 105
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-12028R-Cy3)?
Cy3 on bs-12028R-Cy3: excitation / emission 512,550nm/570,615nm.